Production of non-glycosylated recombinant Pla l 1, the main allergen of Plantago lanceolata
نویسندگان
چکیده
منابع مشابه
Production of non-glycosylated recombinant Pla l 1, the main allergen of Plantago lanceolata
Methods Recombinant Pla l 1.0101 was heterologously expressed in the E. coli strain Rosetta-gami B pLysS and purified using cation exchange and size exclusion chromatography. Natural Pla l 1 was obtained by pollen extraction and cation exchange chromatography. Physico-chemical properties of the purified proteins were analyzed in gel electrophoresis, mass spectrometry and circular dichroism. Usi...
متن کاملMolecular aerobiology - Plantago allergen Pla l 1 in the atmosphere.
INTRODUCTION Exposure to airborne pollen from certain plants can cause allergic disease, but allergens can also be found in non-pollen-bearing fractions of ambient air. This may explain why the allergic response in susceptible patients does not always coincide with the presence and magnitude of airborne pollen counts. Plantago pollen is an important cause of pollinosis in northern Mediterranean...
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ژورنال
عنوان ژورنال: Clinical and Translational Allergy
سال: 2014
ISSN: 2045-7022
DOI: 10.1186/2045-7022-4-s2-p15